Journal: Nature chemistry

Article Title: An automated platform for the enzyme-mediated assembly of complex oligosaccharides

doi: 10.1038/s41557-019-0219-8

Figure Lengend Snippet: a , Sulfonated tags 1a - c bearing an methyl hydroxylamine moiety can be condensed with reducing glycans to give compounds such as 2a-c . After a glycosyltransferase-catalyzed reaction to give compounds such as 3a-c , the sulfonate moieties of the tag allow product purification by DEAE solid phase extraction (SPE). b , Various steps for automated glycosyltransferase-mediated oligosaccharide assembly. The addition of reagents, pH adjustment, withdrawal of samples, transfer of reaction mixtures, and SPE are performed by a 4-needle head mounted on a robotic arm. c , Schematic illustration of the automation platform. A. 4-needle head with pH needle mounted on a robotic arm for liquid handling. B. Four syringe pumps of different capacity to drive liquid handling. C. Temperature controlled shaking rack to hold reaction vessels (50 × 15 mL vials). D. Temperature controlled rack (4 °C) for reagent storage (96 × 8 mL vials). E and F. SPE racks for compound purification (80 × 1 mL cartridges and 80 × 3 mL cartridges). G. Reagent rack (30 × 60 mL vials). H. Sample rack for analysis intermediate compounds (160 × 2 mL vials). I. ISYNTH AI SWING platform.

Article Snippet: A glycosynthesizer was built based on ISYNTH AI SWING workstation from ChemSpeed Technologies ( ).

Techniques: Purification, Extraction

Journal: Nature

Article Title: Autonomous mobile robots for exploratory synthetic chemistry

doi: 10.1038/s41586-024-08173-7

Figure Lengend Snippet: a , Workflow design for autonomous synthesis using mobile robots for sample transport and handling between the ISynth synthesizer and the MS and NMR analysis stations. b , An application-agnostic heuristic reaction planner takes input from the MS and NMR measurements and combines these two orthogonal datasets to plan the next stage in the synthesis experiment. Only reactions that pass predetermined, application-specific thresholds for both the MS and the NMR measurements are deemed to be hits, which are then taken forward for scale-up or for further reproducibility tests (green, pass; orange, fail).

Article Snippet: Apart from the photocatalysis experiments, all synthesis experiments were performed using a Chemspeed ISynth platform.

Techniques: